Transition Metal Ion FRET in the Gas Phase: A 10-40 A Range Molecular Ruler for Mass-Selected Biomolecular Ions
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Open access
Date
2021-08-04Type
- Journal Article
Abstract
Structural studies of mass-selected biomolecules in the gas phase can reveal their intrinsic properties and provide useful benchmarks for biomolecular modeling. Here, we report the first evidence of transition metal ion FRET (tmFRET) in the gas phase and its application to measure short (10-40 A) biomolecular backbone distances. The measured FRET efficiencies in rhodamine dye (donor) labeled helical peptides complexed with Cu2+ ions (acceptor) decreased with increasing donor - acceptor distances, confirming the occurrence of tmFRET. The distances estimated for similar peptide sequences from the FRET efficiencies were consistently longer in the gas phase compared to those reported in solution, indicating an expanded structure and a possible loss of helicity. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000501422Publication status
publishedExternal links
Journal / series
Journal of the American Chemical SocietyVolume
Pages / Article No.
Publisher
American Chemical SocietyOrganisational unit
03430 - Zenobi, Renato / Zenobi, Renato
Funding
ETH-07 15-1 - Native Biomolecular Ions in the Gas Physe? A Functional and Structural Approach (ETHZ)
Related publications and datasets
Is supplemented by: https://doi.org/10.3929/ethz-b-000534351
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