Investigating the intercellular interaction between 3D gut epithelial microtissues and circulating MAIT cells using a microfluidic tilting platform
dc.contributor.author
Nguyen, Thi Phuong Oanh
dc.contributor.author
Misun, Patrick
dc.contributor.author
Lohasz, Christian
dc.contributor.author
Nudischer, Ramona
dc.contributor.author
Frey, Olivier
dc.contributor.author
Devan, Jan
dc.contributor.author
De Libero, Gennaro
dc.contributor.author
Hierlemann, Andreas
dc.contributor.author
Renggli, Kasper
dc.date.accessioned
2021-03-30T07:46:11Z
dc.date.available
2020-01-24T13:38:20Z
dc.date.available
2020-02-14T13:18:15Z
dc.date.available
2021-03-30T07:46:11Z
dc.date.issued
2019
dc.identifier.uri
http://hdl.handle.net/20.500.11850/393846
dc.description.abstract
Microphysiological systems constitute new, versatile tools to investigate the pathogenesis of different immunemediated inflammatory diseases – among them inflammatory bowel disease (IBD). To recapitulate more faithfully the interaction between the gut model and relevant immune cells, we adapted a commercialized microfluidic platform to co-culture three-dimensional (3D) gut epithelial microtissues (MTs) with circulating mucosalassociated invariant T (MAIT) cells in vitro in a dynamic microenvironment. This platform enables i) on-chip optical readout of specific physical interaction between 3D gut epithelial MTs and MAIT cells, and ii) supernatantbased readout of soluble cytokine signals in both, healthy and infection scenarios.
en_US
dc.language.iso
en
en_US
dc.publisher
Chemical and Biological Microsystems Society
en_US
dc.subject
Microphysiological systems
en_US
dc.subject
3D gut epithelial MTs
en_US
dc.subject
MAIT cells
en_US
dc.subject
Microfluidic platform
en_US
dc.title
Investigating the intercellular interaction between 3D gut epithelial microtissues and circulating MAIT cells using a microfluidic tilting platform
en_US
dc.type
Other Conference Item
ethz.book.title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)
en_US
ethz.pages.start
346
en_US
ethz.pages.end
347
en_US
ethz.event
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)
en_US
ethz.event.location
Basel, Switzerland
en_US
ethz.event.date
October 27-31, 2019
ethz.publication.place
San Diego, CA
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
en_US
ethz.date.deposited
2020-01-24T13:38:27Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-02-14T13:18:30Z
ethz.rosetta.lastUpdated
2024-02-02T13:25:14Z
ethz.rosetta.versionExported
true
ethz.COinS
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