Investigating the intercellular interaction between 3D gut epithelial microtissues and circulating MAIT cells using a microfluidic tilting platform
Abstract
Microphysiological systems constitute new, versatile tools to investigate the pathogenesis of different immunemediated inflammatory diseases – among them inflammatory bowel disease (IBD). To recapitulate more faithfully the interaction between the gut model and relevant immune cells, we adapted a commercialized microfluidic platform to co-culture three-dimensional (3D) gut epithelial microtissues (MTs) with circulating mucosalassociated invariant T (MAIT) cells in vitro in a dynamic microenvironment. This platform enables i) on-chip optical readout of specific physical interaction between 3D gut epithelial MTs and MAIT cells, and ii) supernatantbased readout of soluble cytokine signals in both, healthy and infection scenarios. Show more
Publication status
publishedBook title
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)Pages / Article No.
Publisher
Chemical and Biological Microsystems SocietyEvent
Subject
Microphysiological systems; 3D gut epithelial MTs; MAIT cells; Microfluidic platformOrganisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
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