Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast
dc.contributor.author
Wettstein, Rahel
dc.contributor.author
Hugener, Jannik
dc.contributor.author
Gillet, Ludovic
dc.contributor.author
Hernández-Armenta, Yi
dc.contributor.author
Henggeler, Adrian
dc.contributor.author
Xu, Jingwei
dc.contributor.author
van Gerwen, Julian
dc.contributor.author
Wollweber, Florian
dc.contributor.author
Arter, Meret
dc.contributor.author
Aebersold, Ruedi
dc.contributor.author
Beltrao, Pedro
dc.contributor.author
Pilhofer, Martin
dc.contributor.author
Matos, Joao
dc.date.accessioned
2024-07-24T11:37:11Z
dc.date.available
2024-07-07T05:56:22Z
dc.date.available
2024-07-08T08:13:50Z
dc.date.available
2024-07-24T11:37:11Z
dc.date.issued
2024-07-08
dc.identifier.issn
1534-5807
dc.identifier.issn
1878-1551
dc.identifier.other
10.1016/j.devcel.2024.05.025
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/681707
dc.identifier.doi
10.3929/ethz-b-000681707
dc.description.abstract
Sexually reproducing eukaryotes employ a developmentally regulated cell division program—meiosis—to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FoF1-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4ALDH2, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4ALDH2 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Cell Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
meiosis
en_US
dc.subject
cell cycle
en_US
dc.subject
mitochondria
en_US
dc.subject
aldehyde dehydrogenase
en_US
dc.subject
protein filaments
en_US
dc.subject
cryo-electron tomography
en_US
dc.subject
phosphoproteomics
en_US
dc.title
Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2024-06-20
ethz.journal.title
Developmental Cell
ethz.journal.volume
59
en_US
ethz.journal.issue
13
en_US
ethz.journal.abbreviated
Dev Cell
ethz.pages.start
1764
en_US
ethz.pages.end
1782.e8
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::09463 - Pilhofer, Martin / Pilhofer, Martin
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::09758 - Beltrao, Pedro / Beltrao, Pedro
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::09463 - Pilhofer, Martin / Pilhofer, Martin
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::09758 - Beltrao, Pedro / Beltrao, Pedro
ethz.date.deposited
2024-07-07T05:56:22Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2024-07-24T11:37:12Z
ethz.rosetta.lastUpdated
2024-07-24T11:37:12Z
ethz.rosetta.exportRequired
true
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true
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