Engineering Sensory Ganglion Multicellular System to Model Tissue Nerve Ingrowth
Open access
Date
2024-03-20Type
- Journal Article
ETH Bibliography
yes
Altmetrics
Abstract
Discogenic pain is associated with deep nerve ingrowth in annulus fibrosus tissue (AF) of intervertebral disc (IVD). To model AF nerve ingrowth, primary bovine dorsal root ganglion (DRG) micro-scale tissue units are spatially organised around an AF explant by mild hydrodynamic forces within a collagen matrix. This results in a densely packed multicellular system mimicking the native DRG tissue morphology and a controlled AF-neuron distance. Such a multicellular organisation is essential to evolve populational-level cellular functions and in vivo-like morphologies. Pro-inflammatory cytokine-primed AF demonstrates its neurotrophic and neurotropic effects on nociceptor axons. Both effects are dependent on the AF-neuron distance underpinning the role of recapitulating inter-tissue/organ anatomical proximity when investigating their crosstalk. This is the first in vitro model studying AF nerve ingrowth by engineering mature and large animal tissues in a morphologically and physiologically relevant environment. The new approach can be used to biofabricate multi-tissue/organ models for untangling pathophysiological conditions and develop novel therapies. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000650639Publication status
publishedExternal links
Journal / series
Advanced ScienceVolume
Pages / Article No.
Publisher
Wiley-VCHSubject
acoustic assembly; advanced in vitro models; alternatives to animal testing; multicellular system; sensory nerve ingrowthMore
Show all metadata
ETH Bibliography
yes
Altmetrics