Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications
dc.contributor.author
Brosel-Oliu, Sergi
dc.contributor.author
Rius, Gemma
dc.contributor.author
Aviño, Anna
dc.contributor.author
Nakatsuka, Nako
dc.contributor.author
Illa, Xavi
dc.contributor.author
del Corro, Elena
dc.contributor.author
Delgà-Fernández, Marta
dc.contributor.author
Masvidal-Codina, Eduard
dc.contributor.author
Rodríguez, Natalia
dc.contributor.author
Merino, Juan Pedro
dc.contributor.author
Criado, Alejandro
dc.contributor.author
Prato, Maurizio
dc.contributor.author
Tkatchenko, Raphaela
dc.contributor.author
Eritja, Ramón
dc.contributor.author
Godignon, Philippe
dc.contributor.author
Garrido, José Antonio
dc.contributor.author
Villa, Rosa
dc.contributor.author
Guimerà, Anton
dc.contributor.author
Prats-Alfonso, Elisabet
dc.date.accessioned
2024-05-02T13:21:48Z
dc.date.available
2023-12-19T10:34:26Z
dc.date.available
2023-12-22T08:38:06Z
dc.date.available
2024-05-02T13:21:48Z
dc.date.issued
2024-05-02
dc.identifier.issn
1613-6810
dc.identifier.issn
1613-6829
dc.identifier.other
10.1002/smll.202308857
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/648550
dc.identifier.doi
10.3929/ethz-b-000648550
dc.description.abstract
Graphene solution-gated field-effect transistors (gSGFETs) offer high potential for chemical and biochemical sensing applications. Among the current trends to improve this technology, the functionalization processes are gaining relevance for its crucial impact on biosensing performance. Previous efforts are focused on simplifying the attachment procedure from standard multi-step to single-step strategies, but they still suffer from overreaction, and impurity issues and are limited to a particular ligand. Herein, a novel strategy for single-step immobilization of chemically modified aptamers with fluorenylmethyl and acridine moieties, based on a straightforward synthetic route to overcome the aforementioned limitations is presented. This approach is benchmarked versus a standard multi-step strategy using thrombin as detection model. In order to assess the reliability of the functionalization strategies 48-gSGFETs arrays are employed to acquire large datasets with multiple replicas. Graphene surface characterization demonstrates robust and higher efficiency in the chemical coupling of the aptamers with the single-step strategy, while the electrical response evaluation validates the sensing capability, allowing to implement different alternatives for data analysis and reduce the sensing variability. In this work, a new tool capable of overcome the functionalization challenges of graphene surfaces is provided, paving the way toward the standardization of gSGFETs for biosensing purposes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley-VCH
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
array
en_US
dc.subject
biosensor
en_US
dc.subject
chemical functionalization
en_US
dc.subject
field-effect transistor
en_US
dc.subject
graphene
en_US
dc.subject
microfabrication
en_US
dc.subject
solution-gated
en_US
dc.title
Single-Step Functionalization Strategy of Graphene Microtransistor Array with Chemically Modified Aptamers for Biosensing Applications
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2023-12-10
ethz.journal.title
Small
ethz.journal.volume
20
en_US
ethz.journal.issue
18
en_US
ethz.pages.start
2308857
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Graphene Flagship Core Project 3
en_US
ethz.grant
Graphene Flagship Core Project 2
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.grant.agreementno
881603
ethz.grant.agreementno
785219
ethz.grant.fundername
EC
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.grant.program
H2020
ethz.date.deposited
2023-12-19T10:34:33Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2024-05-02T13:21:49Z
ethz.rosetta.lastUpdated
2024-05-02T13:21:49Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Single-Step%20Functionalization%20Strategy%20of%20Graphene%20Microtransistor%20Array%20with%20Chemically%20Modified%20Aptamers%20for%20Biosensing%20Applications&rft.jtitle=Small&rft.date=2024-05-02&rft.volume=20&rft.issue=18&rft.spage=2308857&rft.issn=1613-6810&1613-6829&rft.au=Brosel-Oliu,%20Sergi&Rius,%20Gemma&Avi%C3%B1o,%20Anna&Nakatsuka,%20Nako&Illa,%20Xavi&rft.genre=article&rft_id=info:doi/10.1002/smll.202308857&
Files in this item
Publication type
-
Journal Article [130276]