Multiple roads lead to Rome: unique morphology and chemistry of endospores, exospores, myxospores, cysts and akinetes in bacteria
dc.contributor.author
Corona Ramírez, Andrea
dc.contributor.author
Lee, Kang Soo
dc.contributor.author
Odriozola, Adolfo
dc.contributor.author
Kaminek, Marek
dc.contributor.author
Stocker, Roman
dc.contributor.author
Zuber, Benoît
dc.contributor.author
Junier, Pilar
dc.date.accessioned
2023-03-07T10:02:42Z
dc.date.available
2023-02-28T05:25:29Z
dc.date.available
2023-03-07T10:02:42Z
dc.date.issued
2023-02
dc.identifier.issn
1350-0872
dc.identifier.issn
1465-2080
dc.identifier.other
10.1099/mic.0.001299
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/600779
dc.identifier.doi
10.3929/ethz-b-000600779
dc.description.abstract
The production of specialized resting cells is a remarkable survival strategy developed by many organisms to withstand unfavourable environmental factors such as nutrient depletion or other changes in abiotic and/or biotic conditions. Five bacterial taxa are recognized to form specialized resting cells: Firmicutes, forming endospores; Actinobacteria, forming exospores; Cyanobacteria, forming akinetes; the δ-Proteobacterial order Myxococcales, forming myxospores; and Azotobacteraceae, forming cysts. All these specialized resting cells are characterized by low-to-absent metabolic activity and higher resistance to environmental stress (desiccation, heat, starvation, etc.) when compared to vegetative cells. Given their similarity in function, we tested the potential existence of a universal morpho-chemical marker for identifying these specialized resting cells. After the production of endospores, exospores, akinetes and cysts in model organisms, we performed the first cross-species morphological and chemical comparison of bacterial sporulation. Cryo-electron microscopy of vitreous sections (CEMOVIS) was used to describe near-native morphology of the resting cells in comparison to the morphology of their respective vegetative cells. Resting cells shared a thicker cell envelope as their only common morphological feature. The chemical composition of the different specialized resting cells at the single-cell level was investigated using confocal Raman microspectroscopy. Our results show that the different specialized cells do not share a common chemical signature, but rather each group has a unique signature with a variable conservation of the signature of the vegetative cells. Additionally, we present the validation of Raman signatures associated with calcium dipicolinic acid (CaDPA) and their variation across individual cells to develop specific sorting thresholds for the isolation of endospores. This provides a proof of concept of the feasibility of isolating bacterial spores using a Raman-activated cell-sorting platform. This cross-species comparison and the current knowledge of genetic pathways inducing the formation of the resting cells highlights the complexity of this convergent evolutionary strategy promoting bacterial survival.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Microbiology Society
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
akinete
en_US
dc.subject
calcium dipicolinic acid (CaDPA)
en_US
dc.subject
CEMOVIS
en_US
dc.subject
cyst
en_US
dc.subject
endospore
en_US
dc.subject
exospore
en_US
dc.subject
myxospore
en_US
dc.subject
Raman microspectroscopy
en_US
dc.title
Multiple roads lead to Rome: unique morphology and chemistry of endospores, exospores, myxospores, cysts and akinetes in bacteria
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2023-02-20
ethz.journal.title
Microbiology
ethz.journal.volume
169
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Microbiology (N.Y.)
ethz.pages.start
001299
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Reading
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02115 - Dep. Bau, Umwelt und Geomatik / Dep. of Civil, Env. and Geomatic Eng.::02608 - Institut für Umweltingenieurwiss. / Institute of Environmental Engineering::09467 - Stocker, Roman / Stocker, Roman
ethz.date.deposited
2023-02-28T05:25:31Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2023-03-07T10:02:43Z
ethz.rosetta.lastUpdated
2024-02-02T20:46:29Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Multiple%20roads%20lead%20to%20Rome:%20unique%20morphology%20and%20chemistry%20of%20endospores,%20exospores,%20myxospores,%20cysts%20and%20akinetes%20in%20bacteria&rft.jtitle=Microbiology&rft.date=2023-02&rft.volume=169&rft.issue=2&rft.spage=001299&rft.issn=1350-0872&1465-2080&rft.au=Corona%20Ram%C3%ADrez,%20Andrea&Lee,%20Kang%20Soo&Odriozola,%20Adolfo&Kaminek,%20Marek&Stocker,%20Roman&rft.genre=article&rft_id=info:doi/10.1099/mic.0.001299&
Files in this item
Publication type
-
Journal Article [130276]