The complete genome sequence of Xanthomonas theicola, the causal agent of canker on tea plants, reveals novel secretion systems in clade-1 xanthomonads
Abstract
Xanthomonas theicola is the causal agent of bacterial canker on tea plants. There is no complete genome sequence available for X. theicola, a close relative of the species X. translucens and X. hyacinthi, thus limiting basic research for this group of pathogens. Here, we release a high-quality complete genome sequence for the X. theicola type strain, CFBP 4691T. Single-molecule real-time sequencing with a mean coverage of 264× revealed two contigs of 4,744,641 bp (chromosome) and 40,955 bp (plasmid) in size. Genome mining revealed the presence of nonribosomal peptide synthases, two CRISPR systems, the Xps type 2 secretion system, and the Hrp type 3 secretion system. Surprisingly, this strain encodes an additional type 2 secretion system and a novel type 3 secretion system with enigmatic function, hitherto undescribed for xanthomonads. Four type 3 effector genes were found on complete or partial transposons, suggesting a role of transposons in effector gene evolution and spread. This genome sequence fills an important gap to better understand the biology and evolution of the early-branching xanthomonads, also known as clade-1 xanthomonads. Show more
Publication status
publishedExternal links
Journal / series
PhytopathologyVolume
Pages / Article No.
Publisher
American Phytopathological SocietySubject
Bacterial canker; Bacterial pathogenesis; Effector-encoding transposon; Evolution; Genomics; host parasite interactions; Microbe-genome sequencing; Pathogen effectors; Tea plant; Type 2 secretion; Type 3 secretion; Xanthomonas theicolaOrganisational unit
03969 - Studer, Bruno / Studer, Bruno
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