Quantification of Protein Secretion from Circulating Tumor Cells in Microfluidic Chambers
dc.contributor.author
Armbrecht, Lucas
dc.contributor.author
Rutschmann, Ophélie
dc.contributor.author
Szczerba, Barbara Maria
dc.contributor.author
Nikoloff, Jonas
dc.contributor.author
Aceto, Nicola
dc.contributor.author
Dittrich, Petra S.
dc.date.accessioned
2020-06-12T09:12:32Z
dc.date.available
2020-05-09T03:54:52Z
dc.date.available
2020-05-11T10:30:56Z
dc.date.available
2020-06-12T09:12:32Z
dc.date.issued
2020-06
dc.identifier.issn
2198-3844
dc.identifier.other
10.1002/advs.201903237
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/413984
dc.identifier.doi
10.3929/ethz-b-000413984
dc.description.abstract
Cancer cells can be released from a cancerous lesion and migrate into the circulatory system, from whereon they may form metastases at distant sites. Today, it is possible to infer cancer progression and treatment efficacy by determining the number of circulating tumor cells (CTCs) in the patient's blood at multiple time points; further valuable information about CTC phenotypes remains inaccessible. In this article, a microfluidic method for integrated capture, isolation, and analysis of membrane markers as well as quantification of proteins secreted by single CTCs and CTC clusters is introduced. CTCs are isolated from whole blood with extraordinary efficiencies above 95% using dedicated trapping structures that allow co‐capture of functionalized magnetic beads to assess protein secretion. The patform is tested with multiple breast cancer cell lines spiked into human blood and mouse‐model‐derived CTCs. In addition to immunostaining, the secretion level of granulocyte growth stimulating factor (G‐CSF), which is shown to be involved in neutrophil recruitment, is quantified The bead‐based assay provides a limit of detection of 1.5 ng mL−1 or less than 3700 molecules per cell. Employing barcoded magnetic beads, this platform can be adapted for multiplexed analysis and can enable comprehensive functional CTC profiling in the future.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Breast cancer
en_US
dc.subject
Circulating tumor cells
en_US
dc.subject
CTCs
en_US
dc.subject
G-CSF
en_US
dc.subject
Microfluidics
en_US
dc.subject
Protein secretion
en_US
dc.title
Quantification of Protein Secretion from Circulating Tumor Cells in Microfluidic Chambers
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-04-24
ethz.journal.title
Advanced Science
ethz.journal.volume
7
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
Adv. Sci.
ethz.pages.start
1903237
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Engineering of hybrid cells using lab-on-chip technology
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03807 - Dittrich, Petra / Dittrich, Petra
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09736 - Aceto, Nicola / Aceto, Nicola
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03807 - Dittrich, Petra / Dittrich, Petra
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::09736 - Aceto, Nicola / Aceto, Nicola
ethz.grant.agreementno
681587
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
H2020
ethz.date.deposited
2020-05-09T03:54:57Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-06-12T09:12:42Z
ethz.rosetta.lastUpdated
2022-03-29T02:23:57Z
ethz.rosetta.versionExported
true
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