Imbalanced Regulation of Fungal Nutrient Transports According to Phosphate Availability in a Symbiocosm Formed by Poplar, Sorghum, and Rhizophagus irregularis
dc.contributor.author
Calabrese, Silvia
dc.contributor.author
Cusant, Loic
dc.contributor.author
Sarazin, Alexis
dc.contributor.author
Niehl, Annette
dc.contributor.author
Erban, Alexander
dc.contributor.author
Brulé, Daphnée
dc.contributor.author
Recorbet, Ghislaine
dc.contributor.author
Wipf, Daniel
dc.contributor.author
Roux, Christophe
dc.contributor.author
Kopka, Joachim
dc.contributor.author
Boller, Thomas
dc.contributor.author
Courty, Pierre-Emmanuel
dc.date.accessioned
2020-01-09T13:02:43Z
dc.date.available
2020-01-09T03:27:38Z
dc.date.available
2020-01-09T13:02:43Z
dc.date.issued
2019-12
dc.identifier.issn
1664-462X
dc.identifier.other
10.3389/fpls.2019.01617
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/388722
dc.identifier.doi
10.3929/ethz-b-000388722
dc.description.abstract
In arbuscular mycorrhizal (AM) symbiosis, key components of nutrient uptake and exchange are specialized transporters that facilitate nutrient transport across membranes. As phosphate is a nutrient and a regulator of nutrient exchanges, we investigated the effect of P availability to extraradical mycelium (ERM) on both plant and fungus transcriptomes and metabolomes in a symbiocosm system. By perturbing nutrient exchanges under the control of P, our objectives were to identify new fungal genes involved in nutrient transports, and to characterize in which extent the fungus differentially modulates its metabolism when interacting with two different plant species. We performed transportome analysis on the ERM and intraradical mycelium of the AM fungus Rhizophagus irregularis associated to Populus trichocarpa and Sorghum bicolor under high and low P availability in ERM, using quantitative RT-PCR and Illumina mRNA-sequencing. We observed that mycorrhizal symbiosis induces expression of specific phosphate and ammonium transporters in both plants. Furthermore, we identified new AM-inducible transporters and showed that a subset of phosphate transporters is regulated independently of symbiotic nutrient exchange. mRNA-Sequencing revealed that the fungal transportome was not similarly regulated in the two host plant species according to P availability. Mirroring this effect, many plant carbohydrate transporters were down-regulated in P. trichocarpa mycorrhizal root tissue. Metabolome analysis revealed further that AM root colonization led to a modification of root primary metabolism under low and high P availability and to a decrease of primary metabolite pools in general. Moreover, the down regulation of the sucrose transporters suggests that the plant limits carbohydrate long distance transport (i.e. from shoot to the mycorrhizal roots). By simultaneous uptake/reuptake of nutrients from the apoplast at the biotrophic interface, plant and fungus are both able to control reciprocal nutrient fluxes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
arbuscular mycorrhizal fungi
en_US
dc.subject
symbiocosm
en_US
dc.subject
extraradical mycelium
en_US
dc.subject
intraradical mycelium
en_US
dc.subject
phosphorus
en_US
dc.subject
ammonium
en_US
dc.subject
carbohydrates transporters
en_US
dc.subject
lipid metabolism
en_US
dc.title
Imbalanced Regulation of Fungal Nutrient Transports According to Phosphate Availability in a Symbiocosm Formed by Poplar, Sorghum, and Rhizophagus irregularis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2019-12-12
ethz.journal.title
Frontiers in Plant Science
ethz.journal.volume
10
en_US
ethz.journal.abbreviated
Front. Plant Sci.
ethz.pages.start
1617
en_US
ethz.size
20 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.date.deposited
2020-01-09T03:27:44Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-01-09T13:02:53Z
ethz.rosetta.lastUpdated
2024-02-02T10:06:22Z
ethz.rosetta.versionExported
true
ethz.COinS
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