Self‐Assembly of Proteinaceous Shells around Positively Charged Gold Nanomaterials Enhances Colloidal Stability in High‐Ionic‐Strength Buffers
dc.contributor.author
Sasaki, Eita
dc.contributor.author
Dragoman, Ryan M.
dc.contributor.author
Mantri, Shiksha
dc.contributor.author
Dirin, Dmitry
dc.contributor.author
Kovalenko, Maksym V.
dc.contributor.author
Hilvert, Donald
dc.date.accessioned
2022-03-22T10:09:00Z
dc.date.available
2019-11-28T13:42:36Z
dc.date.available
2019-11-29T12:14:17Z
dc.date.available
2020-02-14T17:26:39Z
dc.date.available
2022-03-22T10:09:00Z
dc.date.issued
2020-01-15
dc.identifier.issn
1439-4227
dc.identifier.issn
1439-7633
dc.identifier.other
10.1002/cbic.201900469
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/381233
dc.description.abstract
The enzyme lumazine synthase (LS) has been engineered to self-assemble into hollow-shell structures that encapsulate unnatural cargo proteins through complementary electrostatic interactions. Herein, we show that a negatively supercharged LS variant can also form organic–inorganic hybrids with gold nanomaterials. Simple mixing of LS pentamers with positively charged gold nanocrystals in aqueous buffer spontaneously affords protein-shelled gold cores. The procedure works well with differently sized and shaped gold nanocrystals, and the resulting shelled complexes exhibit dramatically enhanced colloidal stability over a wide range of pH (4.0–10.0) and at high ionic strength (up to 1 m NaCl). They are even stable over days upon dilution in buffer. Self-assembly of engineered LS shells in this way offers an easy and attractive alternative to commonly used ligand-exchange methods for stabilizing inorganic nanomaterials.
en_US
dc.language.iso
en
en_US
dc.publisher
Wiley
en_US
dc.subject
Colloidal stability
en_US
dc.subject
Gold nanomaterials
en_US
dc.subject
Lumazine synthase
en_US
dc.subject
Proteinaceous shells
en_US
dc.subject
Self-assembly
en_US
dc.title
Self‐Assembly of Proteinaceous Shells around Positively Charged Gold Nanomaterials Enhances Colloidal Stability in High‐Ionic‐Strength Buffers
en_US
dc.type
Journal Article
dc.date.published
2019-09-15
ethz.journal.title
ChemBioChem
ethz.journal.volume
21
en_US
ethz.journal.issue
1-2
en_US
ethz.journal.abbreviated
ChemBioChem
ethz.pages.start
74
en_US
ethz.pages.end
79
en_US
ethz.notes
Published in the Special Issue: "20th Anniversary".
en_US
ethz.grant
Engineered protein capsids as artificial bacterial organelles
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Weinheim
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02513 - Laboratorium für Anorganische Chemie / Laboratory of Inorganic Chemistry::03934 - Kovalenko, Maksym / Kovalenko, Maksym
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02513 - Laboratorium für Anorganische Chemie / Laboratory of Inorganic Chemistry::03934 - Kovalenko, Maksym / Kovalenko, Maksym
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
ethz.grant.agreementno
321295
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2019-11-28T13:42:40Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2020-02-14T17:26:50Z
ethz.rosetta.lastUpdated
2023-02-07T00:26:44Z
ethz.rosetta.versionExported
true
ethz.COinS
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