A Functional General Stress Response of Bradyrhizobium diazoefficiens Is Required for Early Stages of Host Plant Infection
dc.contributor.author
Ledermann, Raphael
dc.contributor.author
Bartsch, Ilka
dc.contributor.author
Müller, Barbara
dc.contributor.author
Wülser, Janine
dc.contributor.author
Fischer, Hans-Martin
dc.date.accessioned
2024-08-19T16:29:08Z
dc.date.available
2018-05-01T02:13:33Z
dc.date.available
2018-05-07T10:54:15Z
dc.date.available
2024-08-19T16:29:08Z
dc.date.issued
2018-05
dc.identifier.issn
0894-0282
dc.identifier.other
10.1094/MPMI-11-17-0284-R
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/261721
dc.description.abstract
Phylogenetically diverse bacteria respond to various stress conditions by mounting a general stress response (GSR) resulting in the induction of protection or damage repair functions. In α-proteobacteria, the GSR is induced by a regulatory cascade consisting of the extracytoplasmic function (ECF) σ factor σEcfG, its anti-σ factor NepR, and the anti-anti-σ factor PhyR. We have reported previously that σEcfG and PhyR of Bradyrhizobium diazoefficiens (formerly named Bradyrhizobium japonicum), the nitrogen-fixing root nodule symbiont of soybean and related legumes, are required for efficient symbiosis; however, the precise role of the GSR remained undefined. Here, we analyze the symbiotic defects of a B. diazoefficiens mutant lacking σEcfG by comparing distinct infection stages of enzymatically or fluorescently tagged wild-type and mutant bacteria. Although root colonization and root hair curling were indistinguishable, the mutant was not competitive, and showed delayed development of emerging nodules and only a few infection threads. Consequently, many of the mutant-induced nodules were aborted, empty, or partially colonized. Congruent with these results, we found that σEcfG was active in bacteria present in root-hair-entrapped microcolonies and infection threads but not in root-associated bacteria and nitrogen-fixing bacteroids. We conclude that GSR-controlled functions are crucial for synchronization of infection thread formation, colonization, and nodule development.
en_US
dc.language.iso
en
en_US
dc.publisher
American Phytopathological Society
en_US
dc.title
A Functional General Stress Response of Bradyrhizobium diazoefficiens Is Required for Early Stages of Host Plant Infection
en_US
dc.type
Journal Article
dc.date.published
2018-03-15
ethz.journal.title
Molecular Plant-Microbe Interactions
ethz.journal.volume
31
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
Mol. Plant-Microb. Interact.
ethz.pages.start
537
en_US
ethz.pages.end
547
en_US
ethz.grant
The role of Bradyrhizobium japonicum extracytoplasmic function (ECF) sigma factor EcfG in formation of an effective symbiosis
en_US
ethz.grant
General stress response of Bradyrhizobium diazoefficiens: signals, signal transduction pathways and effectors involved in symbiosis
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
St. Paul, MN
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08818 - Fischer, Hans-Martin (Tit.-Prof.) (eh.)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::08818 - Fischer, Hans-Martin (Tit.-Prof.) (eh.)
ethz.grant.agreementno
153446
ethz.grant.agreementno
173255
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.grant.program
Projekte Lebenswissenschaften
ethz.date.deposited
2018-05-01T02:13:59Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2018-05-07T10:54:17Z
ethz.rosetta.lastUpdated
2024-02-02T04:42:36Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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Journal Article [130870]