Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways
dc.contributor.author
Müller, Sebastian
dc.contributor.author
Balaz, Miroslav
dc.contributor.author
Stefanicka, Patrik
dc.contributor.author
Varga, Lukas
dc.contributor.author
Amri, Ez-Zoubir
dc.contributor.author
Ukropec, Jozef
dc.contributor.author
Wollscheid, Bernd
dc.contributor.author
Wolfrum, Christian
dc.date.accessioned
2018-10-12T15:10:54Z
dc.date.available
2017-06-12T08:58:34Z
dc.date.available
2018-10-12T15:10:54Z
dc.date.issued
2016-07-15
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/srep30030
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/118302
dc.identifier.doi
10.3929/ethz-b-000118302
dc.description.abstract
Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of primary human brown adipose tissue versus adjacent white adipose tissue, which reveals significant quantitative differences in protein abundances and in turn differential functional capabilities. The majority of the 318 proteins with increased abundance in BAT are associated with mitochondrial metabolism and confirm the increased oxidative capacity. In addition to uncoupling protein 1 (UCP1), the main functional effector for uncoupled respiration, we also detected the mitochondrial creatine kinases (CKMT1A/B, CKMT2), as effective modulators of ATP synthase coupled respiration, to be exclusively expressed in BAT. The abundant expression and utilization of both energy expenditure pathways in parallel highlights the complex functional involvement of BAT in human physiology.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Proteomics
en_US
dc.subject
Preclinical research
en_US
dc.subject
Molecular medicine
en_US
dc.title
Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
6
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
30030
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02072 - Proteomics Plattform D-HEST
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03819 - Wolfrum, Christian / Wolfrum, Christian
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02072 - Proteomics Plattform D-HEST
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03819 - Wolfrum, Christian / Wolfrum, Christian
ethz.date.deposited
2017-06-12T08:59:02Z
ethz.source
ECIT
ethz.identifier.importid
imp5936548a7433694112
ethz.ecitpid
pub:180253
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T10:58:25Z
ethz.rosetta.lastUpdated
2024-02-02T06:20:42Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Proteomic%20Analysis%20of%20Human%20Brown%20Adipose%20Tissue%20Reveals%20Utilization%20of%20Coupled%20and%20Uncoupled%20Energy%20Expenditure%20Pathways&rft.jtitle=Scientific%20Reports&rft.date=2016-07-15&rft.volume=6&rft.spage=30030&rft.issn=2045-2322&rft.au=M%C3%BCller,%20Sebastian&Balaz,%20Miroslav&Stefanicka,%20Patrik&Varga,%20Lukas&Amri,%20Ez-Zoubir&rft.genre=article&rft_id=info:doi/10.1038/srep30030&
Files in this item
Publication type
-
Journal Article [130276]