Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
dc.contributor.author
Mafra, Valéria
dc.contributor.author
Martins, Polyana K.
dc.contributor.author
Francisco, Carolina S.
dc.contributor.author
Ribeiro-Alves, Marcelo
dc.contributor.author
Freitas-Astúa, Juliana
dc.contributor.author
Machado, Marcos A.
dc.date.accessioned
2018-10-22T16:30:24Z
dc.date.available
2017-06-12T01:38:29Z
dc.date.available
2018-10-22T16:30:24Z
dc.date.issued
2013-04-12
dc.identifier.issn
1471-2164
dc.identifier.other
10.1186/1471-2164-14-247
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/113479
dc.identifier.doi
10.3929/ethz-b-000113479
dc.description.abstract
Background
Citrus huanglongbing (HLB) disease is caused by endogenous, phloem-restricted, Gram negative, uncultured bacteria named Candidatus Liberibacter africanus (CaLaf), Ca. L. asiaticus (CaLas), and Ca. L. americanus (CaLam), depending on the continent where the bacteria were first detected. The Asian citrus psyllid vector, Diaphorina citri, transmits CaLas and CaLam and both Liberibacter species are present in Brazil. Several studies of the transcriptional response of citrus plants manifesting HLB symptoms have been reported, but only for CaLas infection. This study evaluated the transcriptional reprogramming of a susceptible genotype of sweet orange challenged with CaLam, using a customized 385K microarray containing approximately 32,000 unigene transcripts. We analyzed global changes in gene expression of CaLam-infected leaves of sweet orange during the symptomatic stage of infection and compared the results with previously published microarray studies that used CaLas-infected plants. Twenty candidate genes were selected to validate the expression profiles in symptomatic and asymptomatic PCR-positive leaves infected with CaLas or CaLam.
Results
The microarray analysis identified 633 differentially expressed genes during the symptomatic stage of CaLam infection. Among them, 418 (66%) were upregulated and 215 (34%) were down regulated. Five hundred and fourteen genes (81%) were orthologs of genes from Arabidopsis thaliana. Gene set enrichment analysis (GSEA) revealed that several of the transcripts encoded transporters associated with the endomembrane system, especially zinc transport. Among the most biologically relevant gene transcripts in GSEA were those related to signaling, metabolism and/or stimulus to hormones, genes responding to stress and pathogenesis, biosynthesis of secondary metabolites, oxidative stress and transcription factors belonging to different families. Real time PCR of 20 candidate genes validated the expression pattern of some genes in symptomatic and asymptomatic leaves infected with CaLam or CaLas.
Conclusions
Many gene transcripts and biological processes are significantly altered upon CaLam infection. Some of them had been identified in response to CaLas infection, while others had not been previously reported. These data will be useful for selecting target genes for genetic engineering to control HLB.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.subject
Gene expression
en_US
dc.subject
Sweet orange
en_US
dc.subject
Huanglongbing
en_US
dc.subject
Plant-pathogen interaction
en_US
dc.title
Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
ethz.journal.title
BMC Genomics
ethz.journal.volume
14
en_US
ethz.journal.abbreviated
BMC Genomics
ethz.pages.start
247
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
ethz.date.deposited
2017-06-12T01:42:32Z
ethz.source
ECIT
ethz.identifier.importid
imp5936542873ad359118
ethz.ecitpid
pub:175171
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T03:40:47Z
ethz.rosetta.lastUpdated
2024-02-02T06:26:13Z
ethz.rosetta.versionExported
true
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