Structural Determinants of Arabidopsis thaliana Hyponastic Leaves 1 Function In Vivo
dc.contributor.author
Burdisso, Paula
dc.contributor.author
Milia, Fernando
dc.contributor.author
Schapire, Arnaldo L.
dc.contributor.author
Bologna, Nicolas G.
dc.contributor.author
Palatnik, Javier F.
dc.contributor.author
Rasia, Rodolfo M.
dc.date.accessioned
2018-10-11T08:41:04Z
dc.date.available
2017-06-11T14:59:25Z
dc.date.available
2018-10-11T08:41:04Z
dc.date.issued
2014-11-19
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0113243
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/95170
dc.identifier.doi
10.3929/ethz-b-000095170
dc.description.abstract
MicroRNAs have turned out to be important regulators of gene expression. These molecules originate from longer transcripts that are processed by ribonuclease III (RNAse III) enzymes. Dicer proteins are essential RNAse III enzymes that are involved in the generation of microRNAs (miRNAs) and other small RNAs. The correct function of Dicer relies on the participation of accessory dsRNA binding proteins, the exact function of which is not well-understood so far. In plants, the double stranded RNA binding protein Hyponastic Leaves 1 (HYL1) helps Dicer Like protein (DCL1) to achieve an efficient and precise excision of the miRNAs from their primary precursors. Here we dissected the regions of HYL1 that are essential for its function in Arabidopsis thaliana plant model. We generated mutant forms of the protein that retain their structure but affect its RNA-binding properties. The mutant versions of HYL1 were studied both in vitro and in vivo, and we were able to identify essential aminoacids/residues for its activity. Remarkably, mutation and even ablation of one of the purportedly main RNA binding determinants does not give rise to any major disturbances in the function of the protein. We studied the function of the mutant forms in vivo, establishing a direct correlation between affinity for the pri-miRNA precursors and protein activity.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
PLOS
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Structural Determinants of Arabidopsis thaliana Hyponastic Leaves 1 Function In Vivo
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
9
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e113243
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
San Francisco, CA
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T15:00:00Z
ethz.source
ECIT
ethz.identifier.importid
imp593652b9d3f1213059
ethz.ecitpid
pub:149349
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T16:11:13Z
ethz.rosetta.lastUpdated
2024-02-02T06:18:15Z
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true
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