Open access
Datum
2014-04-01Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater accuracy and optimal efficiency of recombination. Co-Driver employs a recombinase cascade of Dre and Dre-respondent Cre, which processes loxP-flanked alleles only when both recombinases are expressed in a predetermined temporal sequence. This unique property makes Co-Driver ideal for sequential lineage tracing studies aimed at unraveling the relationships between cellular precursors and mature cell types. Co-InCre was designed for highly efficient intersectional conditional transgenesis. It relies on highly active trans-splicing inteins and promoters with simultaneous transcriptional activity to reconstitute Cre recombinase from two inactive precursor fragments. By generating native Cre, Co-InCre attains recombination rates that exceed all other binary SSR systems evaluated in this study. Both Co-Driver and Co-InCre significantly extend the utility of existing Cre-responsive alleles. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000083668Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Nucleic Acids ResearchBand
Seiten / Artikelnummer
Verlag
Oxford University PressOrganisationseinheit
03742 - Zeilhofer, Hanns U. / Zeilhofer, Hanns U.
Anmerkungen
It was possible to publish this article open access thanks to a Swiss National Licence with the publisherETH Bibliographie
yes
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