Amphotericin B Increases Influenza A Virus Infection by Preventing IFITM3-Mediated Restriction
Open access
Datum
2013-11Typ
- Journal Article
ETH Bibliographie
yes
Altmetrics
Abstract
The IFITMs inhibit influenza A virus (IAV) replication in vitro and in vivo. Here, we establish that the antimycotic heptaen, amphotericin B (AmphoB), prevents IFITM3-mediated restriction of IAV, thereby increasing viral replication. Consistent with its neutralization of IFITM3, a clinical preparation of AmphoB, AmBisome, reduces the majority of interferon’s protective effect against IAV in vitro. Mechanistic studies reveal that IFITM1 decreases host-membrane fluidity, suggesting both a possible mechanism for IFITM-mediated restriction and its negation by AmphoB. Notably, we reveal that mice treated with AmBisome succumbed to a normally mild IAV infection, similar to animals deficient in Ifitm3. Therefore, patients receiving antifungal therapy with clinical preparations of AmphoB may be functionally immunocompromised and thus more vulnerable to influenza, as well as other IFITM3-restricted viral infections. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000078523Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Cell ReportsBand
Seiten / Artikelnummer
Verlag
ElsevierOrganisationseinheit
03511 - Carreira, Erick M. / Carreira, Erick M.
ETH Bibliographie
yes
Altmetrics