Liquid Chromatographic Isolation of Individual Amino Acids Extracted From Sediments for Radiocarbon Analysis
Abstract
The “building blocks of life” are found nearly ubiquitously in the environment in the form of proteins, peptides, and single amino acids. To shed light on amino acid sources, cycling, and preservation in sedimentary environments, we present a method using high-pressure liquid chromatography to separate and isolate underivatized amino acids extracted from sediments to conduct compound-specific radiocarbon analysis. This method consists of three main steps including (1) amino acid extraction by hydrofluoric and hydrochloric acids followed by desalting, (2) liquid chromatographic isolation and purification using two complementary column chemistries, and (3) post-purification and measurement by accelerator mass spectrometry. The resulting blank of this procedure is estimated to contain 2.2 ± 1.3 μgC with 0.25 ± 0.09 Fm. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000408854Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Frontiers in Marine ScienceBand
Seiten / Artikelnummer
Verlag
Frontiers MediaThema
14C; compound-specific radiocarbon analysis; demineralization; hydrolysis; desalting; protein; peptide; purificationOrganisationseinheit
08619 - Labor für Ionenstrahlphysik (LIP) / Laboratory of Ion Beam Physics (LIP)
03868 - Eglinton, Timothy I. / Eglinton, Timothy I.