Vascular endothelial growth factor a and VEGFR-1 change during preimplantation in heifers
dc.contributor.author
Chiumia, Daniel
dc.contributor.author
Hankele, Anna-Katharina
dc.contributor.author
Groebner, Anna E.
dc.contributor.author
Schulke, Katy
dc.contributor.author
Reichenbach, Horst-Dieter
dc.contributor.author
Giller, Katrin
dc.contributor.author
Zakhartchenko, Valeri
dc.contributor.author
Bauersachs, Stefan
dc.contributor.author
Ulbrich, Susanne E.
dc.date.accessioned
2020-02-04T10:20:41Z
dc.date.available
2020-02-02T14:12:57Z
dc.date.available
2020-02-04T10:20:41Z
dc.date.issued
2020-01
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms21020544
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/396515
dc.identifier.doi
10.3929/ethz-b-000396515
dc.description.abstract
Vascular endothelial growth factor A (VEGFA) plays a critical angiogenic role in the endometrium of placentalia during preimplantation. The role of VEGFA and its receptors is not fully characterised in bovine reproduction. We analysed the mRNA expression of VEGFA isoforms 121, 165 and 189, and VEGF receptors 1 and 2 in three experimental settings (A, B and C). We compared intercaruncular endometrium of cyclic to pregnant heifers at Days 12, 15 and 18 post insemination (Day 0), and between Day 15 and Day 18 conceptuses (A). We further compared caruncular versus intercaruncular endometrium at Day 15 (B), and endometrium of heifers carrying embryos originating from somatic cell nuclear transfer (SCNT) versus in vitro fertilisation (IVF) at Day 18 (C). Endometrial VEGFA protein was localised and quantified. Pregnant heifers displayed lower intercaruncular endometrial mRNA expression of VEGFA-121 (p = 0.045) and VEGFA-189 (p = 0.009) as well as lower VEGFA protein abundance (p < 0.001) at Day 15. The VEGFA protein was localised in intercaruncular luminal, glandular epithelium and in tunica muscularis of blood vessels. At Day 15, caruncular endometrium displayed higher VEGFA mRNA expression than intercaruncular endometrium (p < 0.05). Intercaruncular endometrial VEGFA protein at Day 18 was higher in abundance in SCNT than in IVF (p = 0.038). Therefore, during preimplantation in cattle, there may be a need for timely physiological reduction in intercaruncular endometrial VEGFA expression in favour of the caruncular area to facilitate a gradient towards the implantation sites. A higher expression of VEGFA in SCNT may predispose for later placentation abnormalities frequently observed following SCNT.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
angiogenesis
en_US
dc.subject
bovine
en_US
dc.subject
conceptus
en_US
dc.subject
endometrium
en_US
dc.subject
IVF
en_US
dc.subject
SCNT
en_US
dc.title
Vascular endothelial growth factor a and VEGFR-1 change during preimplantation in heifers
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2020-01-15
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
21
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.pages.start
544
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03428 - Kreuzer, Michael (emeritus) / Kreuzer, Michael (emeritus)
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03428 - Kreuzer, Michael (emeritus) / Kreuzer, Michael (emeritus)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
ethz.relation.isPartOf
10.3929/ethz-b-000397239
ethz.date.deposited
2020-02-02T14:13:00Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2020-02-04T10:20:52Z
ethz.rosetta.lastUpdated
2022-03-29T00:55:04Z
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true
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Journal Article [130141]