Rolling Circle Transcription-Amplified Hierarchically Structured Organic-Inorganic Hybrid RNA Flowers for Enzyme Immobilization
![Thumbnail](/bitstream/handle/20.500.11850/354767/acsami.9b04663.pdf.jpg?sequence=6&isAllowed=y)
Open access
Autor(in)
Alle anzeigen
Datum
2019-07-03Typ
- Journal Article
ETH Bibliographie
yes
Altmetrics
Abstract
Programmable nucleic acids have emerged as powerful building blocks for the bottom-up fabrication of two- or three-dimensional nano- and microsized constructs. Here we describe the construction of organic–inorganic hybrid RNA flowers (hRNFs) via rolling circle transcription (RCT), an enzyme-catalyzed nucleic acid amplification reaction. These hRNFs are highly adaptive structures with controlled sizes, specific nucleic acid sequences, and a highly porous nature. We demonstrated that hRNFs are applicable as potential biological platforms, where the hRNF scaffold can be engineered for versatile surface functionalization and the inorganic component (magnesium ions) can serve as an enzyme cofactor. For surface functionalization, we proposed robust and straightforward approaches including in situ synthesis of functional hRNFs and postfunctionalization of hRNFs that enable facile conjugation with various biomolecules and nanomaterials (i.e., proteins, enzymes, organic dyes, inorganic nanoparticles) using selective chemistries (i.e., avidin–biotin interaction, copper-free click reaction). In particular, we showed that hRNFs can serve as soft scaffolds for β-galactosidase immobilization and greatly enhance enzymatic activity and stability. Therefore, the proposed concepts and methodologies are not only fundamentally interesting when designing RNA scaffolds or RNA bionanomaterials assembled with enzymes but also have significant implications on their future utilization in biomedical applications ranging from enzyme cascades to biosensing and drug delivery. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000354767Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
ACS Applied Materials & InterfacesBand
Seiten / Artikelnummer
Verlag
American Chemical SocietyThema
Rolling circle transcription; RNA flowers; Organic−inorganic hybrid structures; Enzyme immobilization; Enzymatic study; Allosteric effectFörderung
701994 - Exponential Amplification and Rapid Detection of miRNAs using DNA-Quantum Dot Bioconjugates for Disease Diagnostics (EC)
ETH Bibliographie
yes
Altmetrics