Proteomic Analysis of Human Brown Adipose Tissue Reveals Utilization of Coupled and Uncoupled Energy Expenditure Pathways
Abstract
Human brown adipose tissue (BAT) has become an attractive target to combat the current epidemical spread of obesity and its associated co-morbidities. Currently, information on its functional role is primarily derived from rodent studies. Here, we present the first comparative proteotype analysis of primary human brown adipose tissue versus adjacent white adipose tissue, which reveals significant quantitative differences in protein abundances and in turn differential functional capabilities. The majority of the 318 proteins with increased abundance in BAT are associated with mitochondrial metabolism and confirm the increased oxidative capacity. In addition to uncoupling protein 1 (UCP1), the main functional effector for uncoupled respiration, we also detected the mitochondrial creatine kinases (CKMT1A/B, CKMT2), as effective modulators of ATP synthase coupled respiration, to be exclusively expressed in BAT. The abundant expression and utilization of both energy expenditure pathways in parallel highlights the complex functional involvement of BAT in human physiology. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000118302Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Scientific ReportsBand
Seiten / Artikelnummer
Verlag
NatureThema
Proteomics; Preclinical research; Molecular medicineOrganisationseinheit
02072 - Proteomics Plattform D-HEST
03819 - Wolfrum, Christian / Wolfrum, Christian