The environmental stress response regulates ribosome content in cell cycle-arrested S. cerevisiae
Abstract
Prolonged cell cycle arrests occur naturally in differentiated cells and in response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether and how cells survive prolonged cell cycle arrests is not clear. Here, we used S. cerevisiae to compare physiological cell cycle arrests and genetically induced arrests in G1-, meta- and anaphase. Prolonged cell cycle arrest led to growth attenuation in all studied conditions, coincided with activation of the Environmental Stress Response (ESR) and with a reduced ribosome content as determined by whole ribosome purification and TMT mass spectrometry. Suppression of the ESR through hyperactivation of the Ras/PKA pathway reduced cell viability during prolonged arrests, demonstrating a cytoprotective role of the ESR. Attenuation of cell growth and activation of stress induced signaling pathways also occur in arrested human cell lines, raising the possibility that the response to prolonged cell cycle arrest is conserved. Show more
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https://doi.org/10.3929/ethz-b-000613520Publication status
publishedExternal links
Journal / series
Frontiers in Cell and Developmental BiologyVolume
Pages / Article No.
Publisher
Frontiers MediaSubject
environmental stress response (ESR); cell cycle arrest; cytoplasm dilution; ribosome fraction; growth lawOrganisational unit
09713 - Neurohr, Gabriel / Neurohr, Gabriel
Funding
187003 - The impact of cell size on cell function in physiology and aging (SNF)
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Is supplemented by: https://doi.org/10.3929/ethz-b-000602269
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