The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival
dc.contributor.author
Steffen, Philipp A.
dc.contributor.author
Altmutter, Christina
dc.contributor.author
Dworschak, Eva
dc.contributor.author
Junttila, Sini
dc.contributor.author
Gyenesei, Attila
dc.contributor.author
Zhu, Xinzhou
dc.contributor.author
Kockmann, Tobias
dc.contributor.author
Ringrose, Leonie
dc.date.accessioned
2021-09-13T13:27:23Z
dc.date.available
2021-08-10T03:01:21Z
dc.date.available
2021-09-13T12:59:08Z
dc.date.available
2021-09-13T13:27:23Z
dc.date.issued
2021
dc.identifier.issn
0009-5915
dc.identifier.issn
1432-0886
dc.identifier.other
10.1007/s00412-021-00762-z
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/500204
dc.identifier.doi
10.3929/ethz-b-000500204
dc.description.abstract
The Drosophila Trithorax group (TrxG) protein ASH1 remains associated with mitotic chromatin through mechanisms that are poorly understood. ASH1 dimethylates histone H3 at lysine 36 via its SET domain. Here, we identify domains of the TrxG protein ASH1 that are required for mitotic chromatin attachment in living Drosophila. Quantitative live imaging demonstrates that ASH1 requires AT hooks and the BAH domain but not the SET domain for full chromatin binding in metaphase, and that none of these domains are essential for interphase binding. Genetic experiments show that disruptions of the AT hooks and the BAH domain together, but not deletion of the SET domain alone, are lethal. Transcriptional profiling demonstrates that intact ASH1 AT hooks and the BAH domain are required to maintain expression levels of a specific set of genes, including several involved in cell identity and survival. This study identifies in vivo roles for specific ASH1 domains in mitotic binding, gene regulation, and survival that are distinct from its functions as a histone methyltransferase.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
ASH1
en_US
dc.subject
Trithorax
en_US
dc.subject
Chromatin
en_US
dc.subject
Drosophila
en_US
dc.subject
Mitosis
en_US
dc.title
The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2021-07-01
ethz.journal.title
Chromosoma
ethz.journal.volume
130
en_US
ethz.journal.issue
2
en_US
ethz.pages.start
215
en_US
ethz.pages.end
234
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Berlin
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2021-08-10T03:01:49Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2021-09-13T12:59:17Z
ethz.rosetta.lastUpdated
2022-03-29T11:54:24Z
ethz.rosetta.versionExported
true
ethz.COinS
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